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Buy 0.1 retin a queous cream, and 1.5 mL of 0.05% ketoconazole solution. A drop of 25 g/L solution p-aminobenzoic acid was added in the 0.1% retin oil and mixture was stirred for 5 minutes at room temperature. A drop of the 0.05% ketoconazole solution was added in a 50 mL/L ratio. After adding the retin oil, mixture was stirred for 5 minutes at room temperature and then extracted with saturated sodium hydroxide solution (20 mL/L) and methanol. The organic layer was dried over anhydrous Na 2 SO 4 and concentrated to dryness by sulphuric acid solution. The residue was purified by column chromatography eluting with hexane (70:30) to afford 0.1% retin aqueous cream. EXAMPLE 4.1 Aqueous suspension of p-aminobenzoic acid (20 mg/mL), 1–2 g/L of p-aminobenzoic acid in aqueous retin oil (1–2 mL/L), 1 mL of 1.7 g/L ethyl p-coumaric acid, and 3.2 mL of 2.3 g/L ethyl p-coumarate in methanol were added to a mixture of 20 mL 3 mL/L aqueous retin oil and 50 mL of methanol. After 3 hours agitation, this suspension was transferred (5 mL/L) onto a glass vial containing 25 mL of a 0.1% retin oil solution and 5 mL of the 2.3 g/L ethyl p-coumarate solution in methanol. The vials were then shaken vigorously (15 min) and then the liquids were removed under nitrogen. The residue was collected and decanted. The residue was purified by column chromatography eluting with hexane (70:30) to afford 0.2% retin acyl acid. The effect of aqueous retin oil on keratinocyte differentiation was examined by flow cytometry following the procedure of Dehghani et al (2005). Flow cytometry was performed as described previously using 4-8% retin oil solution and 1% H 2 O in methanol. The flow cytometer used was a BD FACSAria II (BD Biosciences, San Jose, CA). EXAMPLE 4.2 Aqueous suspension of p-aminobenzoic acid (20 Is generic atorvastatin as good as brand-name lipitor mg/mL), 1–2 g/L of p-aminobenzoic acid in aqueous retin oil (1–2 mL/L), 1 mL of 1.7 g/L ethyl p-coumaric acid, and 3.2 mL of 2.3 g/L ethyl p-coumarate in methanol were added to a mixture of 20 mL 3 mL/L aqueous retin oil, 50 mL of methanol, and 4% glycerol (diluted 3:1) in a microcentrifuge tube containing 5 mL of acetonitrile. The solution was agitated for 48 hours (15 minutes at room temperature) and then dried over anhydrous Na 2 SO 4. The suspension and remaining acetonitrile (2 mL) were dissolved in 1N HCl (10 mL) and the solution was refluxed Esomeprazol online bestellen for 48 hours. The acetonitrile solution was filtered pharmacy online coupon and the filtrate washed with 20% ethanol and methanol finally the residue was purified by column chromatography eluting with hexane (70:30) to afford 0.2% p-aminobenzoic acid. In a further study, the effect of retinol on keratinocyte differentiation was examined by flow cytometry. cytometry was performed as described previously using 4-8% retin oil solution, 1% H 2 O in methanol, and 20% glycerol a microcentrifuge tube containing 5 mL of acetonitrile. The solution was agitated for 48 hours (15 minutes at room temperature) and then dried over anhydrous Na 2 SO 4. The suspension and remaining acetonitrile (2 mL) were dissolved in 1N HCl (10 mL) and the solution was refluxed for 48 hours. The acetonitrile solution was filtered and the filtrate washed with 20% ethanol and methanol finally the residue was purified by column chromatography eluting with hexane (70:30) to afford 0.4% retinol. EXAMPLE 4.3

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